Table 1 Deduced functions of ORFs involving bafilomycin biosynthesis in K. setae KM-6054, S. griseus DSM 2608 and S. lohii JCM 14114

From: Characterization of bafilomycin biosynthesis in Kitasatospora setae KM-6054 and comparative analysis of gene clusters in Actinomycetales microorganisms

K. setae KM-6054			S. griseus DSM 2608			S. lohii JCM 14114
ORF	aa ^a	Predicted function	ORF	aa ^a	Homology ^b	ORF	aa ^a	Homology ^b	Homology ^c
BfmM	538	Acyl-CoA ligase	BafBI^d	461	88/93	BafX	528	87/91	93/95
BfmL	517	Amide synthase	BafBII	514	86/90	BafY	514	86/90	95/96
BfmK	410	5-Aminolevulinate synthase	BafBIII	414	87/92	BafZ	414	88/93	97/98
BfmA1	4833	Modular PKS Loading module: AT(iB)^e-ACP Module 1: KS-AT (mM)^e-KR (B)^f-ACP Module 2: KS-AT (M)^e-KR (A)^f-ACP Module 3: KS-AT (mM)^e-ACP	BafSI	5015	84/88	BafAI	4884	82/86	92/94
BfmA2	5004	Modular PKS Module 4: KS-AT (mM)^e-KR (A)^f-ACP Module 5: KS-AT (Mo)^e-KR (B)^f-ACP Module 6: KS-AT (M)^e-DH-KR(B)^f-ACP	BafSII	5088	84/88	BafAII	5145	86/90	94/95
BfmA3	3964	Modular PKS Module 7: KS-AT (mM)^e-DH-KR (B)^f-ACP Module 8: KS-AT (mM)^e-DH-ER-KR (B)^f-ACP	BafSIII	3980	84/89	BafAIII	3968	85/89	93/95
BfmA4	3436	Modular PKS Module 9: KS-AT (mM)^e-KR (A)^f-ACP Module 10: KS-AT (mM)^e-DH-KR (B)^f-ACP	BafSIV	3626	83/87	BafAIV	3511	84/89	89/91
BfmA5	2110	Modular PKS Module 11: KS-AT (Mo)^e-DH-KR (B)^f-ACP-TE	BafSV	2105	84/89	BafAV	2103	83/87	93/95
BfmB	301	3-Hydroxyacyl-CoA dehydrogenase^g	BafAI	296	89/93	BafB	296	89/93	96/98
BfmC	93	ACP^g	BafAII	93	82/88	BafC	93	86/91	96/96
BfmD	371	Acyl-CoA dehydrogenase^g	BafAIII	363	90/93	BafD	363	89/93	97/98
BfmE	383	Hydrolase^g	BafAIV	366	91/95	BafE	365	91/95	97/98
BfmF	220	O-methyltransferase^g	BafAV	220	90/96	BafF	220	90/96	95/98
BfmR	622	Transcriptional regulator (DnrI, RedD, AfsR-family)	BafRI	609	83/87	BafG	609	84/87	94/96
BfmG	253	TE	BafT	253	81/88	BafH	253	82/88	96/96
BfmH	105	Transcriptional regulator (LuxR-family)	BafRII	117	72/78	Orf1	117	73/79	92/94
BfmI	320	AT	Orf17	348	88/93	Orf2	320	88/93	96/98
BfmJ	363	AMP-binding enzyme	Orf18	359	88/92	Orf3	332	89/92	96/98

Abbreviations: A, A-type; ACP, acyl carrier protein; AT, acyltransferase;, B, B-type; DH, dehydratase; ER, enoyl reductase; iB, isobutyryl-ACP extender; KR, β-ketoacyl-ACP reductase; KS, β-ketoacyl-ACP synthase; M, malonyl-ACP extender; mM, methylmalonyl-ACP extender; Mo, methoxymalonyl-ACP extender; ORF, open reading frame; TE, thioesterase.
^aNumber of amino acids.
^bHomology to the ORF of K. setae KM-6054 (% identity/similarity).
^cHomology to the ORF of S. griseus DSM 2608 (% identity/similarity).
^dPartial sequence.
^ePredicted substrate specificity of AT: iB, M, mM anf Mo.
^fPredicted stereochemistry type of KR: A and B.
^gThese genes were involved in the biosynthesis of methoxymalonyl-ACP.

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Table 1 Deduced functions of ORFs involving bafilomycin biosynthesis in K. setae KM-6054, S. griseus DSM 2608 and S. lohii JCM 14114

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