Summary
Qualitative and quantitative differences between the EsD 7 and EsD 1 subunits are analysed by two-dimensional gel electrophoresis. The molecular weight of the EsD 7 subunit is slightly smaller than that of the EsD 1 subunit. However, the pI of the EsD 7 subunit is indistinguishable from that of EsD 1 subunit. In the heterozygote with EsD 7-1, the amount of the EsD 7 subunit is very similar to that of the EsD 1 subunit. The results indicate that EsD 7 is a molecular weight variant and coded for by a new allele,EsD 7, distinct fromEsD 1 at EsD locus. The data also suggest that the amount of theEsD 7 product is similar to that of theEsD 1 product but that the specific activity of EsD 7 is very low compared with that of EsD 1.
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Kondo, I., Nishigaki, I., Yamakawa, K. et al. The esterase D polymorphism: Analysis of esterase D 7 by two-dimensional gel electrophoresis. Jap J Human Genet 29, 27–30 (1984). https://doi.org/10.1007/BF01876754
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DOI: https://doi.org/10.1007/BF01876754
