Summary
We have identified a polymorphic 9-bp repeat sequence in exon 1 of thehMSH3 gene using polymerase chain reaction (PCR). Five alleles were observed in unrelated Japanese individuals with heterozygosity of 0.57.
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References
Anagnou NP, Antonarakis SE, O'Brien SJ, Modi WS, Nienhuis AW (1988): Chromosomal localization and racial distribution of the polymorphic human dihydrofolate reductase pseudogene (DHFRP1). Am J Hum Genet42:345–352
Chakraborty R, Smouse PE Neel JV (1988): Population amalgation and genetic variation: observations on artificially agglomerated tribal populations of Central and South America. Am J Hum Genet43:709–725
Chen M-J, Shimada T, Moulton AD, Cline A, Humphries RK, Maizel J, Nienhuis AW (1984): The functional human dihydrofolate reductase gene. J Biol Chem259:3933–3943
Fishel R, Lescoe MK, Rao MRS, Copeland NG, Jenkins NA, Garber J, Kane M, Kolodner R (1993): The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer. Cell75:1027–1038
Fujii H, Shimada T (1989): Isolation and characterization of cDNA clones derived from the divergently transcibed gene in the region upstream from the human dihydrofolate reductase gene. J Biol Chem264:10057–10064
Leach FS, Nicolaides NC, Papadopoulos N, Liu B, Jen J, Parsons R, Peltomäki P, Sistonen P, Aaltonen LA, Nyström-Lahti M, Guan X-Y, Zhang J Meltzer PS, Yu J-W, Kao F-T, Chen DJ, Cerosaletti KM, Fournier REK, Todd S, Lewis T, Leach RJ, Naylor SL, Weissenbach J, Mecklin J-P, Järvinen H, Petersen GM, Hamilton SR, Green J, Jass J, Watson P, Lynch HT, Trent JM, de la Chapelle A, Kinzer KW, Vogelstein B (1993): Mutations of a MutS homolog in hereditary nonpolyposis colorectal cancer. Cell75:1215–1225
New L, Liu K, Crouse GF (1993): The yeast gene MSH3 defines a new class of eukaryotic MutS homologues. Mol Gen Genet239:97–108
Parsons R, Li G-M, Longley MJ, Fang W-h, Papadopoulos N, Jen J, de la Chapelle A, Kinzler KW, Vogelstein B, Modrich P (1993): Hypermutability and mismatch repair deficiency in RER+ tumor cells. Cell75:1227–1236
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Nakajima, E., Orimo, H., Ikejima, M. et al. Nine-bp repeat polymorphism in exon 1 of thehMSH3 gene. Jap J Human Genet 40, 343–345 (1995). https://doi.org/10.1007/BF01900603
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DOI: https://doi.org/10.1007/BF01900603
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