Summary
A reliable method for obtaining high-resolution R-banded chromosomes from lymphoblastoid cell lines is described. The cell cultures are subjected to S-phase synchronization in the presence of excess thymidine (300 μg/ml) for 17 to 19 hr, followed by BrdU treatment (30 μg/ml) for 6.5 hr. Prior to harvest, they are exposed to ethidium bromide (7.5 μg/ml) for 1.5 hr and Colcemid (0.02 μg/ml) for 30 min. Using this method, high-resolution R-banded chromosomes at the 550–850 band level were obtained with frequencies at high as 70% of all mitotic cells.
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Ye, M.H., Saito-Ohara, F. & Ikeuchi, T. High-resolution chromosome R-banding in lymphoblastoid cell lines by the combined use of cell synchronization and ethidium bromide treatment. Jap J Human Genet 41, 203–208 (1996). https://doi.org/10.1007/BF01892628
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DOI: https://doi.org/10.1007/BF01892628
