Abstract
Loss of heterozygosity (LOH) involving chromosome 16q23.4 occurs frequently in breast tumors, which suggests that this region may contain a tumor suppressor gene. Since ZFP276 is located in this region, we have therefore cloned and performed mutation analysis of its coding region in 70 breast tumors. One silent polymorphism and two alterations predicted to result in amino acid changes were detected. Absence of the wild-type allele in tumors carrying the E530D variant suggests a possible role for this change in tumorigenesis.
Similar content being viewed by others
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
References
Brenner AJ, Aldaz CM (1997) The genetics of sporadic breast cancer. Prog Clin Biol Res 396:63–82
Chen T, Sahin A, Aldaz CM (1996) Deletion map of chromosome 16q in ductal carcinoma in situ of the breast: refining a putative tumor suppressor gene region. Cancer Res 56:5605–5609
Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
Cleton-Jansen AM, Moerland EW, Kuipers-Dijkshoorn NJ, Callen DF, Sutherland GR, Hansen B, Devilee P, Cornelisse CJ (1994) At least two different regions are involved in allelic imbalance on chromosome arm 16q in breast cancer. Genes Chromosomes Cancer 9:101–107
Cleton-Jansen AM, Callen DF, Seshadri R, Goldup S, McCallum B, Crawford J, Powell JA, Settasatian C, van Beerendonk H, Moerland EW, Smit VTBHM, Harris WH, Millis R, Morgan NV, Barnes D, Mathew CG, Cornelisse CJ (2001) Loss of heterozygosity mapping at chromosome arm 16q in 712 breast tumors reveals factors that influence delineation of candidate regions. Cancer Res. 61:1171–1177
Gokgoz N, Wunder JS, Mousses S, Eskandarian S, Bell RS, Andrulis IL (2001) Comparison of p53 mutations in patients with localized osteosarcoma and metastatic osteosarcoma. Cancer 92:2181–2189
Lumb NJ, Danpure CJ (2000) Functional synergism between the most common polymorphism in human alanine: glyoxylate aminotransferase and four of the most common disease-causing mutations. J Biol Chem 275:36415–36422
Savino M, d’Apolito M, Centra M, van Beerendonk HM, Cleton-Jansen AM, Whitmore SA, Crawford J, Callen DF, Zelante L, Savoia A (1999) Characterization of copine VII, a new member of the copine family, and its exclusion as a candidate in sporadic breast cancers with loss of heterozygosity at 16q24.3. Genomics 61:219–226
Shen LX, Basilion JP, Stanton VP Jr (1999) Single-nucleotide polymorphisms can cause different structural folds of mRNA. Proc Natl Acad Sci USA 6:7871–7876
Strathdee CA, Gavish H, Shannon WR, Buchwald M (1992) Cloning of cDNAs for Fanconi’s anaemia by functional complementation. Nature 356:763–767
Whitmore SA, Settasatian C, Crawford J, Lower KM, McCallum B, Seshadri R, Cornelisse CJ, Moerland EW, Cleton-Jansen AM, Tipping AJ, Mathew CG, Savnio M, Savoia A, Verlander P, Auerbach AD, Van Berkel C, Pronk JC, Doggett NA, Callen DF (1998) Characterization and screening for mutations of the growth arrest- specific 11 (GAS11) and C16orf3 genes at 16q24.3 in breast cancer. Genomics 52:325–331
Wong JC, Alon N, Norga K, Kruyt FA, Youssoufian H, Buchwald M (2000) Cloning and analysis of the mouse Fanconi anemia group A cDNA and an overlapping penta zinc finger cDNA. Genomics 67:273–283
Acknowledgements
We thank the pathologists and surgeons of Mount Sinai Hospital for specimens. This work was supported by the Canadian Institutes of Health Research (CIHR), the Lombard Insurance Chair in Pediatrics Research, and the Canadian Breast Cancer Research Initiative (ICA).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wong, J.C.Y., Gokgoz, N., Alon, N. et al. Cloning and mutation analysis of ZFP276 as a candidate tumor suppressor in breast cancer. J Hum Genet 48, 668–671 (2003). https://doi.org/10.1007/s10038-003-0088-1
Received:
Accepted:
Published:
Issue date:
DOI: https://doi.org/10.1007/s10038-003-0088-1
