Abstract
Tol2 is an active DNA-based transposable element identified in the medaka fish, Oryzias latipes. Originating from a vertebrate and belonging to the hAT (hobo/Activator/Tam3) transposable element family, featuring a wide distribution among organisms, Tol2 would be expected to be active if introduced into mammals. We, therefore, examined if excision, one part of the transposition reaction, can occur in human and mouse culture cells. A Tol2 clone was introduced into cells and, after incubation, recovered. PCR and sequencing analysis provided evidence for precise and near precise excision in these cells. Tol2 can thus be expected to serve as a material for developing a gene transfer vector and other genetic tools applicable to mammals. It was also suggested that an intact Tol2 element could retain autonomy as a transposable element in mammalian cells.
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References
Calvi BR, Hong TJ, Findley SD, Gelbert WM (1991) Evidence for a common evolutionary origin of inverted repeat transposons in Drosophila and plants: hobo, Activator, and Tam3. Cell 66:465–471
Dupuy AJ, Clark K, Carlson CM, Fritz S, Davidson AE, Markley KM, Finley K, Fletcher CF, Ekker SC, Hackett PB, Horn S, Largaespada DA (2002) Mammalian germ-line transgenesis by transposition. Proc Natl Acad Sci USA 99:4495–4499
Fedoroff N, Wessler S, Shure M (1983) Isolation of the transposable maize controlling elements Ac and Ds. Cell 35:235–242
Finnegan DJ (1992) Transposable elements. Curr Opin Genet Dev 2:861–867
Fischer SE, Wienholds E, Plasterk RH (2001) Regulated transposition of a fish transposon in the mouse germ line. Proc Natl Acad Sci USA 98:6759–6564
Horie K, Kuroiwa A, Ikawa M, Okabe M, Kondoh G, Matsuda Y, Takeda J (2001) Efficient chromosomal transposition of a Tc1/mariner-like transposon Sleeping Beauty in mice. Proc Natl Acad Sci USA 98:9191–9196
Ivics Z, Hackett PB, Plasterk RH, Izsvák Z (1997) Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell 91:501–510
Izsvák Z, Ivics Z, Plasterk RH (2000) Sleeping Beauty, a wide host-range transposon vector for genetic transformation in vertebrates. J Mol Biol 302:93–102
Kempken F, Windhofer F (2001) The hAT family: a versatile transposon group common to plants, fungi, animals, and man. Chromosoma 110:1–9
Klinakis AG, Zagoraiou L, Vassilatis DK, Savakis C (2000) Genome-wide insertional mutagenesis in human cells by the Drosophila mobile element Minos. EMBO Rep 1:416–421
Koga A, Hori H (2000) Detection of de novo insertion of the medaka fish transposable element Tol2. Genetics 156:1243–1247
Koga A, Hori H (2001) The Tol2 transposable element of the medaka fish: an active DNA-based element naturally occurring in a vertebrate genome. Genes Genet Syst 76:1–8
Koga A, Suzuki M, Inagaki H, Bessho Y, Hori H (1996) Transposable element in fish. Nature 383:30
Koga A, Suzuki M, Maruyama Y, Tsutsumi M, Hori H (1999) Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences. FEBS Lett 461:295–298
Koga A, Hori H, Sakaizumi M (2002) Gene transfer and cloning of flanking chromosomal regions using the medaka fish Tol2 transposable element. Mar Biotechnol 4:6–11
Kozak M (1987) An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res 15:8125–8148
Kunze R (1996) The maize transposable element Activator (Ac). In: Saedler H, Gierl A (eds) Transposable elements. Springer, Berlin, pp 161–194
O'Hare K, Rubin GM (1983) Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome
Pohlman RF, Fedoroff NV, Messing J (1984) The nucleotide sequence of the maize controlling element Activator. Cell 37:635–643
Schouten GJ, van Luenen HG, Verra NC, Valerio D, Plasterk RH (1998) Transposon Tc1 of the nematode Caenorhabditis elegans jumps in human cells. Nucleic Acids Res 26:3013–3017
Wirtz U, Osborne B, Baker B (1997) Ds excision from extrachromosomal geminivirus vector DNA is coupled to vector DNA replication in maize. Plant J 11:125–135
Yant SR, Meuse L, Chiu W, Ivics Z, Izsvák Z, Kay MA (2000) Somatic integration and long-term transgene expression in normal and haemophilic mice using a DNA transposon system. Nat Genet 25:35–41
Zagoraiou L, Drabek D, Alexaki S et al. (2001) In vivo transposition of Minos, a Drosophila mobile element, in mammalian tissues. Proc Natl Acad Sci USA 98:11474–11478
Zhang L, Sankar U, Lampe DJ, Robertson HM Graham FL (1998) The Himar1 mariner transposase cloned in a recombinant adenovirus vector is functional in mammalian cells. Nucleic Acids Res 26:3687–3693
Acknowledgements
We are grateful to D.L. Hartl, S. Hamada and M. Moore for helpful discussion. The HeLa cells were obtained from the Health Science Research Resources Bank of the Japan Health Sciences Foundation. This work was supported by grant no. 10216205 to A.K. and no. 10216206 to A.T. from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Basic Science Research Grant from the Sumitomo Foundation to A.K.
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Koga, A., Iida, A., Kamiya, M. et al. The medaka fish Tol2 transposable element can undergo excision in human and mouse cells. J Hum Genet 48, 231–235 (2003). https://doi.org/10.1007/s10038-003-0016-4
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DOI: https://doi.org/10.1007/s10038-003-0016-4
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