Figure 5

(a, b) Western blotting analysis of NF-κB p65 in cytosol and nuclear extracts of untreated WT and IL-1ra KO mice. Representative results from six independent experiments shown are in (a). The ratios of NF-κB p65 to β-actin were calculated and are shown in (b). All values represent the mean±s.e.m. (n=6 animals). ^*P<0.05, WT vs IL-1ra KO mice. (c) DNA-binding activity of the nuclear NF-κB p65 in the livers of WT and IL-1ra KO mice before and after APAP treatment (200 mg/kg). All values represent the mean±s.e.m. (n=6 animals). ^*P<0.05, WT vs IL-1ra KO mice.