Figure 2
From: Intraocular expression and release of high-mobility group box 1 protein in retinal detachment
Immunofluorescence analysis of HMGB1 in a rat model of RD. Representative photomicrographs of retinal sections labeled with anti-HMGB1 antibody (red; a–c) and DAPI (blue; d–f). Merged images (g–i) are also presented. The retinal sections were derived from the control eye (a, d, g), those at 3 days (day 3; b, e, h), or 7 days after RD (day 7; c, f, i). Arrows point to retinal pigment epithelium (a, g), and arrowheads indicate subretinal macrophages (b, c, h, i). Note that expression of HMGB1 is augmented especially in ONL at day 3 after RD, whereas the upregulation in ONL appears to be subside by day 7 (n=6 for each time point). Scale bars: 50 μm. INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer.
