Figure 2
From: Genomic analysis of the HER2/TOP2A amplicon in breast cancer and breast cancer cell lines
Frequency of amplifications and deletions on chromosome 17 in 15 HER2-amplified breast cancers (a) and in the subset (n=10) of HER2/TOP2A-co-amplified breast cancers (b). The proportion of tumours in which each clone is amplified (green bars) or lost (red bars) is plotted (X axis) for each BAC clone according to genomic location (Y axis). Horizontal dotted lines represent chromosome centromeres. The smallest regions of overlap and the genes mapping to these regions in HER2-amplified cancers and HER2/TOP2A-co-amplified breast cancers are highlighted on the right. In red, genes whose expression is reported to correlate with gene amplification.14, 15, 16, 17, 18, 21 (c) Representative chromosome 17 plots of five HER2-amplified breast cancer cell lines. Note that out of these cell lines, only SKBR3 and UACC812 harboured HER2/TOP2A co-amplification. MDA-MB-361, MDA-MB-453 and ZR-75.30 displayed deletions of the TOP2A gene. Log2 ratios are plotted on the X axis against each clone according to genomic location on the Y axis. The centromere is represented by a horizontal dotted line. HER2 and TOP2A amplicons are highlighted by dark and light grey boxes, respectively.
