Table 1 Summary of cases subjected to chromogenic in situ hybridisation and microarray-based comparative genomic hybridisation

Case	Type	Grade^a	ER/PR^b	HER2 CISH	HER2 aCGH	TOP2A CISH	TOP2A aCGH	qRT-PCR
A11735P	MIMP	3	−ve/−ve	Amp	Amp	Not Amp	Not Amp	Yes
M13	IDC	3	+ve/−ve	Amp	Amp	Not Amp^c	Not Amp	Yes
M324	IDC	3	+ve/+ve	Amp	Amp	Not Amp	Not Amp	Yes
M345	IDC	3	+ve/+ve	Amp	Amp	Not Amp	Not Amp	Yes
M348	IDC	3	−ve/−ve	Amp	Amp	Not Amp	Not Amp	Yes
M37	IDC	3	+ve/−ve	Amp	Amp	Amp	Amp	Yes
M61	P-ILC	3	+ve/−ve	Amp	Amp	Amp	Amp	Yes
M166	IDC	3	+ve/−ve	Amp	Amp	Amp	Amp	Yes
M168	IDC	3	+ve/+ve	Amp	Amp	Amp	Amp	Yes
M180	IDC	3	−ve/−ve	Amp	Amp	Amp	Amp	Yes
M275	IDC	3	+ve/+ve	Amp	Amp	Amp	Amp	Yes
M300	IDC	2	+ve/+ve	Amp	Amp	Amp	Amp	Yes
M309	IDC	3	−ve/−ve	Amp	Amp	Amp	Amp	Yes
M312	Mixed	3	+ve/+ve	Amp	Amp	Amp	Amp	Yes
X644	MIMP	3	−ve/−ve	Amp	Amp	Amp	Amp	Yes
M346	Mixed	2	+ve/+ve	Amp	NA	Not Amp	NA	Yes
M144	IDC	3	−ve/+ve	Amp	NA	Not Amp	NA	Yes
M347	IDC	3	+ve/+ve	Amp	NA	Not Amp	NA	Yes
M327	IDC	3	−ve/+ve	Amp	NA	Not Amp	NA	Yes
M19	IDC	3	+ve/+ve	Amp	NA	Not Amp	NA	Yes
M230	IDC	3	+ve/+ve	Amp	NA	Not Amp	NA	Yes
M259	IDC	3	+ve/+ve	Amp	NA	Not Amp	NA	Yes
M257	IDC	3	−ve/+ve	Amp	NA	Amp	NA	Yes
M316	IDC	3	+ve/+ve	Amp	NA	Amp	NA	Yes
M337	IDC	2	+ve/+ve	Amp	NA	Amp	NA	Yes
M245	IDC	3	+ve/+ve	Amp	NA	Amp	NA	Yes
M270	IDC	2	−ve/−ve	Amp	NA	Amp	NA	Yes

^aBloom–Richardson–Scarff histological grade.
^bAs defined by an Allred score ≥3.
^cThis case showed low-level copy ‘amplification’ using only the TOP2A probe. When ratios TOP2A:CEP17 were devised, the case lacked TOP2A gene amplification.
−ve, negative; +ve, positive.
aCGH, microarray-based comparative genomic hybridisation; Amp, amplified; CISH, chromogenic in situ hybridisation; IDC, invasive ductal carcinoma of no special type; MIMP, mixed invasive micropapillary carcinoma; mixed, mixed invasive ductal and lobular carcinoma; not Amp, not amplified; NA, not assessable due to failed quality control PCR; P-ILC, pleomorphic lobular carcinoma.

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