Figure 2 | Laboratory Investigation

Figure 2

From: Chitinase 3-like-1 enhances bacterial adhesion to colonic epithelial cells through the interaction with bacterial chitin-binding protein

Figure 2

Effect of CBP21 expression in adhesion rate of S. marcescens in CECs. WT, CBP21 knockout and CBP21-restored CBP21 knockout strains of S. marcescens was used for bacterial adhesion assay. SW480 cells were infected with the S. marcescens for 2 h at an MOI of 20. CBP21 knockout strain of S. marcescens significantly decreased its adhesion to SW480 cells compared with WT strain of S. marcescens. In contrast, after restoring the CBP21 expression by merging CBP21 knockout strain of S. marcescens and CBP21-overexpressing PMMB67EH/HB101 E. coli, the bacteria significantly increased the adhesion rate compared with the CBP21 knockout strain of S. marcescens, but almost similar adhesion as WT strain of S. marcescens. Each value is the mean±s.e.m. Three separate experiments. NS, no significant difference, *P<0.05, **P<0.01.

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