Figure 5 | Laboratory Investigation

Figure 5

From: Chitinase 3-like-1 enhances bacterial adhesion to colonic epithelial cells through the interaction with bacterial chitin-binding protein

Figure 5

Generation of point mutant forms of CBP21-overexpressing E. coli and adhesion rate of the E. coli to CECs. Adhesion assays were performed after infecting with pRSET empty (control) vector-, pRSET CBP21 WT (WT CBP21) vector-, pRSET CBP21 mutant forms of six residues to alanine (Y54A, E55A, E60A, H114A, D182A and N185A) vector- or pRSET CBP21 3 combined mutant form of Y54A, E55A and E60A (3MU) vector-transformed BL21-AI strain of E. coli. All groups of bacteria were cultured with 1 mM IPTG and 0.2% L-arabinose for 2 h. BL21 strain of E. coli with a single mutation of either Y54 or E55 residue of CBP21 exhibited a significantly decreased binding to SW480 cells compared with WT CBP21 E. coli. The binding ability was 74% reduced by the 3MU compared with WT CBP21 E. coli. Each value is the mean±s.e.m. of at least three separate experiments. *P<0.05, **P<0.01.

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