Figure 2 | Laboratory Investigation

Figure 2

From: Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas

Figure 2

Bisulfite genomic sequencing (BGS) DNA methylation analysis of the CST6 promoter in normal brain and cultured cells. (a) Schematic of the CST6 promoter region with the vertical tick marks indicating CpG sites and the bent arrow (+1) denoting the transcription start site defined using NCBI Map Viewer. Below this, the boundary of the regions analyzed by BGS, MSP, and pyrosequencing throughout the paper are shown with numbering relative to the transcription start site. (b) BGS analysis of normal white matter and cortex tissues, NHA, and human oligodendrocyte precursor cells (HOPC). The total percent methylation for all clones is indicated at the left. The BGS region shown in part (a) is expanded at the top. Each row of circles (representing CpG sites) corresponds to one sequenced clone. Open and filled circles represent unmethylated and methylated CpGs, respectively.

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