Table 2 Summary of CST6 expression and methylation in pediatric brain tumor primary cell lines

From: Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas

Tumor type/sample ID

Age

Sex

Expressiona

DNA methylationb

Glioma

 106G (grade II)

15

F

−

+

 143G (grade II)

12

F

−

−

 150G (grade III)

12

M

−

+

 157G (grade II)

15

M

−

+

Ependymoma

 147E

14

F

−

+

 168E

5

M

−

+

 169E

3

M

−

+

Pilocytic astrocytoma

 93PA

5

M

−

−

 101PA

15

M

−

±

 122PA

9

F

−

−

 129PA

5

M

−

±

 134PA

1

M

−

−

Totals

  

12/12 (100%)

8/12 (66%)

  1. aReduced or absent in untreated cells and upregulated by 5-azadC treatment.
  2. bPresence of amplification with the ‘M’-specific primers in PCR, ‘±’ weak but higher than normals.