Figure 3 | Laboratory Investigation

Figure 3

From: Post-exposure targeting of specific epitopes on ricin toxin abrogates toxin-induced hypoglycemia, hepatic injury, and lethality in a mouse model

Figure 3

Migration of leukocytes to liver after ricin challenge in vivo. (a and c) Immunoreactive neutrophils in peri-portal (a) and centro-lobular (c) areas of liver 48 h after toxin administration. (b) Quantification of neutrophils as number of 7/4+ immunoreactive cells per high-powered field 0–48 h after toxin was given. (d and f) Immunoreactive macrophages in peri-portal (d) and centro-lobular (f) hepatic areas 2 h after toxin challenge. (e) Number of F4/80+ immunoreactive cells per HPF of liver 0--48 h after administration of toxin. Repeat studies showed loss of stained cells occurred over 8–12 h. Findings are representative of two independent studies with a total of four mice per time point. Magnification: × 400 (a and c); × 200 (d and f). P<0.01, number of leukocytes per HPF at the designated hours compared with that at 0 h. P<0.02, number of macrophages per HPF at 0 h compared with that at 2 h in the peri-portal area.

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