Figure 4 | Laboratory Investigation

Figure 4

From: Rho/ROCK and MEK/ERK activation by transforming growth factor-α induces articular cartilage degradation

Figure 4

Histology and immunostaining for aggrecan and type II collagen cleavage fragments. Osteochondral explants were cultured for 5 days with vehicle (control) or 10 ng/ml TGFα in the presence or absence of a ROCK (Y27632), MEK (U0126), p38 MAPK (SB202190) or PI3K (LY294002) inhibitor (10 μM). Tissue sections were stained with safranin-O/fast green or immunostained for MMP-generated aggrecan or type II collagen cleavage fragments (brown stain). All sections were counterstained with hematoxylin. Arrowhead indicates chondrocyte cluster. Scale bars represent 50 μm.

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