Figure 2 | Laboratory Investigation

Figure 2

From: Detergent-resistant microdomains mediate activation of host cell signaling in response to attaching–effacing bacteria

Figure 2

Activated phosphoinositide 3-kinase (PI3K) is recruited to lipid rafts in response to infection, independent of the type III secretion system and Shiga-like toxins 1 and 2. Western blots of PI3K from ultracentrifugation fractions of whole-cell (HEp-2) extracts. PI3K recruitment in EHEC mutant (CVD 451 and 85-170)-infected cells indicates that signaling is independent of the type III secretion system and Shiga-like toxins 1 and 2 (a). Cells treated with the PI3K inhibitor LY294002 before EHEC O157:H7 infection had reduced amounts of PI3K recruited to lipid rafts, compared with the amount of PI3K recruited in cells treated with the inactive analog, LY303511, before EHEC infection (b).

Back to article page