Table 1 Primer sequences for bisulfite sequencing PCR of hepatic Gck, L-PK and Glut2 and pyrosequencing of Gck promoter

From: Rapid quantification of DNA methylation by measuring relative peak heights in direct bisulfite-PCR sequencing traces

Gene

Forward primer (5′ → 3′)

Reverse primer (5′ → 3′)

Bisulfite sequencing PCR (BSP)

  

Gck 1st

GAATTTTATAGAAGAGTTTAGAATGTTTTGG

CACACCTTATAATATCCATAACCATCTC

Gck 2nd

GGGTGTTAGGGTAGTTAGAGGATTTG

CCTAACTCCTAAAACCACCTATTAC

LPK

TTTTGTGGGGTAGGATGTTTAATATAG

TTCCAACTCCAAACAATTCTCTAAC

Glut2

GGGGATTGTGAAGATTGATAAGTAG

TTCTCAAAAACACTCAAAAATTCCTA

Pyrosequencing of Gck

  

Pyr

TATGGTGAAGTTTTAGATTGTGTGA

Biotin-AAATAACTAAAACCTACCCACTCCC

Pyrs

TGAAGTTTTAGATTGTGTGA

 
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