Figure 4 | Laboratory Investigation

Figure 4

From: Local overexpression of interleukin-1 family, member 6 relates to the development of tubulointerstitial lesions

Figure 4

Localizations of IL-1F6 in the kidneys of CGN mice. (a) An IL-1F6-positive epithelial cell of the DCT tended to drop out to the tubular lumen in the kidney of a 12-month-old female B6.MRLc1 mouse. Bar=20 μm. (b) In the IL-1F6-positive CCD, some positive epithelia showed strong reactions. Bar=20 μm. (c, d) In some IL-1F6-positive tubules, the epithelium transformed to the squamous morphology accompanied by elongation of tubular lumina. Mononuclear cell infiltration was evident around these tubules. Bar=20 μm. (e) IL-1F6-positive cells (TRITC, red) did not show colocalization with the Hnf4α-positive proximal tubular cells (FITC, green) (Figure 3e). Bar=20 μm. (f–h) Post-embedding immunoelectron microscopic analysis revealed IL-1F6-positive signals (10-nm particles) in both the cytoplasm and nucleus (f, arrows). The gold particles were mainly localized on free ribosomes in the cytoplasm (g). In some renal tubules, gold particles were found to localize in vesicles at the apex of epithelium and tubular lumen (h). N=nucleus, L=lumen of renal tubule. Bar=200 nm.

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