Figure 6

Blood glucose, urine glucose, and IL-1F6 protein levels, and Il1f6 mRNA expressions in STZ-induced diabetic mice. (a, b) Time course of blood (a) and urine (b) glucose levels in the citrate buffer (CB) or STZ-administered group. Each value is expressed as mean (s.e.); ^*: significantly different from mice in the CB group for each week (Mann–Whitney U-test, P<0.05); n=4. ND: In mice belonging to the CB group, urinary glucose was not detected. (c, d) IHC staining for IL-1F6 in the kidneys of STZ-administered mice at 15 weeks of age. For mice with mild changes in the renal histopathology, a few renal tubular epithelia that transformed to squamous morphology showed an IL-1F6-positive reaction (c). For mice with numerous dilated renal tubules, the number of IL-1F6-positive tubules remarkably increased (d). Bar=50 μm. (e, f) The number of IL-1F6-positive tubules (e) and the levels of Il1f6 mRNA expression (f) in the kidneys of mice belonging to the CB or STZ-administered group. Each value is expressed as mean (s.e.); ^*: significantly different from mice belonging to the CB group for each graph (Mann–Whitney U-test, P<0.05); n=4.