Figure 9
In vitro analysis of changes in the expression of various mRNAs and morphology with Il1f6 inductions in M-1 cells. (a) Real-time PCR analysis for Il1f6 mRNA expressions in M-1 cells derived from murine collecting ducts and stimulated with 10 or 100 μl of serum (upper lane) and urine (middle lane) of C57BL/6 and MRL/lpr mice or spleen cells (lower lane) of C57BL/6 mice. (b) Quantitative analysis of Il1f6 mRNA expressions stimulated by spleen cells. Each value is expressed as mean (s.e.). (c–e) Quantitative analysis for mRNA expressions of Il1f6, IL-6 (Il6), TGF-β (tgfb) family (c), and various epithelial (d) or mesenchymal (e) markers in M-1 cells stimulated by spleen cells. Each value is expressed as mean (s.e.) and represents the fold increase in the non-stimulated control. (f, g) Morphological changes in M-1 cells on collagen gel after stimulation with spleen cells (arrows). Non-stimulated M-1 cells show cuboidal to squamous epithelium and regular paving morphology (f). Stimulated M-1 cells transformed to squamous morphology, and cellular adhesions tended to loosen (g). Bar=20 μm. PAS stain.
