Table 1 Summary of gene-specific primer pairs
Gene name | Symbol | Accession no. | Forward primer | Reverse primer | Product size (bp) | Application |
|---|---|---|---|---|---|---|
Actin, α2, smooth muscle, aorta | Acta2 | NM_007392.2 | 5′-CAGCACCATGAAGATCAAGATC-3′ | 5′-CGTTCACAGTTGTGTGCTAGAG-3′ | 201 | Real-time PCR |
Actin, β | Actb | NM_007393.3 | 5′-TGTTACCAACTGGGACGACA-3′ | 5′-GGGGTGTTGAAGGTCTCAAA-3′ | 165 | RT–PCR, Real-time PCR |
Cadherin-1 | Cdh1 | NM_009864.2 | 5′-ACGTCCATGTGTGTGACTGTG-3′ | 5′-AGGAGCAGCAGGATCAGAATC-3′ | 139 | Real-time PCR |
Claudin-3 | Cldn3 | NM_009902.3 | 5′-GCACCCACCAAGATCCTCTA-3′ | 5′-TCGTCTGTCACCATCTGGAA-3′ | 206 | Real-time PCR |
Fibronectin-1 | Fn1 | NM_010233.1 | 5′-TGACAACTGCCGTAGACCTG-3′ | 5′-TCGTCTCTGTCAGCTTGCAC-3′ | 162 | Real-time PCR |
Interleukin-1 family, member-6 | Il1f6 | NM_019450.3 | 5′-GAGCAAACAGTTCCAGTCAC-3′ | 5′-ATCTTGAGAGAGTGCCACAG-3′ | 408 | RT–PCR in organs or cells |
5′-CAGCATCACCTTCGCTTAGAC-3′ | 5′-AGTGTCCAGATATTGGCATGG-3′ | 143 | RT–PCR in urine, Real-time PCR | |||
Interleukin-6 | Il6 | NM_031168.1 | 5′-AGCCAGAGTCCTTCAGAGAG-3′ | 5′-GCCACTCCTTCTGTGACTCC-3′ | 140 | Real-time PCR |
Keratin-8 | Krt8 | NM_031170.2 | 5′-GGAGATGGCCATTAAGGATG-3′ | 5′-TGTTCTGCATCCCAGACTCC-3′ | 200 | Real-time PCR |
Matrix metallopeptidase-9 | Mmp9 | NM_013599.2 | 5′-TGGGTGTACACAGGCAAGAC-3′ | 5′-ACTCCTTATCCACGCGAATG-3′ | 199 | Real-time PCR |
Nestin | Nes | NM_016701.3 | 5′-GCTGGAACAGAGATTGGAAGG | 5′-CATCTTGAGGTGTGCCAGTTG-3′ | 157 | Real-time PCR |
Occludin | Ocln | NM_008756.2 | 5′-AGAGTACATGGCTGCTGCTG-3′ | 5′-CCACCATCCTCTTGATGTGC-3′ | 128 | Real-time PCR |
S100 calcium binding protein-A4 | S100a4 | NM_011311.1 | 5′-CAGGCAAAGAGGGTGACAAG-3′ | 5′-GCAATGCAGGACAGGAAGAC-3′ | 190 | Real-time PCR |
Transforming growth factor, β1 | Tgfb1 | NM_011577.1 | 5′-TTGCTTCAGCTCCACAGAGA-3′ | 5′-TGGTTGTAGAGGGCAAGGAC-3′ | 183 | Real-time PCR |
Transforming growth factor, β receptor-1 | Tgfbr1 | NM_009370.2 | 5′-ACCTTCTGATCCATCGGTTG-3′ | 5′-TTCCTGTTGGCTGAGTTGTG-3′ | 202 | Real-time PCR |
Tight junction protein-1 (zona occludens-1) | Tjp1 | NM_009386.1 | 5′-GCACCATGCCTAAAGCTGTC-3′ | 5′-ACTCAACACACCACCATTGC-3′ | 122 | Real-time PCR |
Tight junction protein-2 (zona occludens-2) | Tjp2 | NM_004817.2 | 5′-AATGCGAGGATCGAAATAGC-3′ | 5′-TAGCTTCCTCTGGTGTCCTG-3′ | 158 | Real-time PCR |
Vimentin | Vim | NM_011701.4 | 5′-CAGGATTTCTCTGCCTCTGC-3′ | 5′-TCAAGGTCATCGTGATGCTG-3′ | 171 | Real-time PCR |
