Figure 7 | Laboratory Investigation

Figure 7

From: Ah receptor represses acute-phase response gene expression without binding to its cognate response element

Figure 7The alternative text for this image may have been generated using AI.

AHR-mediated repression of SAA in human cells. (a) Real-time PCR on RNA from Huh7 cells treated with TCDD (10 nM, 30 min) followed by human interleukins (2 ng/ml each of IL1B and IL6) for 6 h. Human SAA1 mRNA abundance was assayed. (b) ELISA to quantify SAA protein secreted by Huh7 cells, treated for 10 h or 24 h with TCDD and interleukins. Just before treatment, cells were transferred to serum-free medium. (c and d) siRNA-driven AHR knock down in Huh7 cells. 36 h after siRNA transfection, cells were treated with TCDD and interleukins, as in (a). SAA1 mRNA (c) and CYP1A1 (d) mRNA levels were determined by real-time PCR. (e) An alternate representation of data from (c). SAA1 induction, on interleukin exposure of scrambled and anti-AHR siRNA-transfected cells, was scaled to 100 units. (f) Real-time PCR to assess SAA1 mRNA induction in Huh7 cells electroporated with a different anti-AHR siRNA oligo (Dharmafect). Data represent mean induction±s.e.m. (n=3/treatment group) and were analyzed to determine significance (*P<0.05; **P<0.01).

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