Figure 3 | Laboratory Investigation

Figure 3

From: Interleukin-18 predicts atherosclerosis progression in SIV-infected and uninfected rhesus monkeys (Macaca mulatta) on a high-fat/high-cholesterol diet

Figure 3

Serial sections of abdominal aorta demonstrating pro-IL-18 production in atherosclerotic lesions. (a) Immunolabeling for CD68 (brown) and pro-IL-18 (pink) results in such extensive overlap in macrophage-rich regions of atherosclerotic plaques that very little differentiation of individuated signal is possible. (CD68 immunohistochemistry with DAB chromogen, pro-IL-18 immunohistochemistry with Fast Red chromogen, and Mayer's hematoxylin counterstain.) (b) Pseudofluorescent unmixed representation of the relative contributions of each chromogen in the stained tissue in panel (a), with pro-IL-18 (green), CD68 (red), and colocalized signal (yellow). Pro-IL-18 signal is extensively colocalized with CD68, but regions of individuated CD68 signal beyond the areas of colocalization are present. (Spectral imaging of chromogens from immunohistochemistry in a.) (c) Immunolabeling for CD3 (brown) and pro-IL-18 (pink) yields clearly differentiable signal for each (CD3 immunohistochemistry with DAB chromogen, pro-IL-18 immunohistochemistry with Fast Red chromogen, and Mayer's hematoxylin). (d) Pseudofluorescent unmixed representation of the relative contributions of each chromogen in the stained tissue in panel (a), with pro-IL-18 (green), and CD3 (red). Pro-IL-18 and CD3 labels show no colocalization (yellow) and occupy clearly distinct regions in the tissue. (Spectral imaging of chromogens from immunohistochemistry in (c).)

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