Figure 2

SHP-1 protein expression in macrophages of MS patients compared to normal subjects (a) SHP-1 protein levels of MS patients and normal subjects were measured with intracellular flow cytometry. The histograms are labeled as follows: shaded histogram for isotype control, solid line for normal subject, and dashed line for MS patient. (b) SHP-1 expression was measured by flow cytometry and quantified using the mean fluorescence intensity (MFI). SHP-1 levels from 24 normal subjects and 27 MS patients were normalized to average SHP-1 levels in normal subjects represented as 1. (c) Western blot analysis of SHP-1 and actin in macrophages MS patients compared to normal subjects. (d) SHP-1 protein in macrophages of MS patients and normal subjects measured by western immunoblot was quantified by measuring the pixel density.