Figure 9
From: Genistein induces adipogenesis but inhibits leptin induction in human synovial fibroblasts
JAK-2 tyrosine kinase inhibitor mimics the anti-leptin effect of genistein and genistein inhibits prednisolone-induced tyrosine phosphorylation. (a) Synovial fibroblasts were stimulated with genistein (50 μM) or JAK-2 inhibitor tyrphostin AG 490 (50 μM) in the presence or absence of prednisolone (1 μM) for 1 week. ELISA measured leptin production in cell supernatant. *A, statistically different from prednisolone-treated cells. (b) Synovial fibroblasts were stimulated with genistein (50 μM), dexamethasone (1 μM), prednisolone (1 μM) or with genistein and each glucocorticoid simultaneously for 3 weeks. Western blot shows tyrosine phosphorylation in total synovial fibroblast extracts. ERK 1/2 expression was used as control.
