Figure 1
From: Tumor suppressor FOXO3 participates in the regulation of intestinal inflammation
TNFα induces FOXO3 translocation in HT-29 cells. (a) HT-29 cells, control and treated with TNFα were fixed, immunofluorescent stained for FOXO3 and images were taken with matched exposures. In control cells, FOXO3 is localized in the nucleus. During TNFα treatment, nuclear FOXO3 translocates to the cytosol, suggesting its inactivation ( × 60 magnification). This experiment was repeated three independent times. (b) Nuclear (8 μg) and cytosolic (40 μg) proteins from HT-29 cells, control and TNFα treated for 3 h, were separated on SDS-PAGE and immunoblotted for FOXO3. Immunoblots reveal decreased nuclear and increased cytosolic amounts of FOXO3 after TNFα treatment. Immunoblots were reprobed with antibodies against Oct-1 for nuclear extracts and actin for cytosolic extracts as a control. Densitometric analysis shows a significant differences (*) between groups (P<0.05).
