Figure 3
From: Tumor suppressor FOXO3 participates in the regulation of intestinal inflammation
TNFα-induced FOXO3 phosphorylation is PI3K dependent. The HT-29 cells; with or without pretreatment with PI3K inhibitor (LY294002) were incubated with TNFα. Protein was separated on SDS-PAGE and immunoprobed with an antibody against phosphorylated FOXO3 at the Thr32 PI3K-dependent site and actin. The immunoblot is representative of three independent experiments (three samples were used per experimental group). Densitometric analysis shows a significant increase (*) in phosphorylated FOXO3 after TNFα treatment, which is attenuated in the presence of LY294002 (**) (P<0.05).
