Figure 7

Foxo3 deficiency leads to severe intestinal inflammation. Foxo3 knockout (KO) and wild-type (WT) mice (n=7 from each group) were treated with 2.5% DSS for 5 days and left 2 days to recover. (a) Measuring blood in stool revealed that KO mice have more bleeding than WT mice (P<0.05). (b) Graph represents inflammation scoring index between KO and WT mice (P<0.05). (c) H&E staining revealed mild inflammation in WT and severe inflammation in KO colon ( × 20 magnification: bar 100 μm). (d and e) Graphs represent the number of lymphocytes and PMNs in colonic epithelia enumerated in five different fields. Asterisk represents significant differences between WT and KO mice (P<0.05).