Figure 3
From: TLR2 expression and signaling-dependent inflammation impair wound healing in diabetic mice
(a) Representative western blot showing expression of MyD88, pIRAK-1, TRIF, and TRAF-6 in day 1 and day 10 wound tissue lysates (8 mm) immunoprecipitated with TLR2 antibody as detailed in Materials and methods. β-Actin was used as a loading control, n=6 mice per group. (b) The DNA-binding activity of nuclear NF-κB p65 in day 1 and day 10 wound tissues (8 mm) was assessed by ELISA as detailed in Materials and methods. Values are normalized to mg nuclear protein and expressed as mean±s.d. *P<0.01 vs C57BL/6J; n=8 mice per group. (c) Lipid peroxidation in wound tissue (8 mm) lysates was measured using thiobarbituric acid reactive substances (TBARS) assay as described in Materials and methods. Values are normalized to mg cell protein and expressed as mean±s.d. *P<0.01 vs C57BL/6J; **P<0.05 vs C57BL/6J+STZ; n=8 mice per group.
