Figure 5
From: Inhibition of matrix metalloproteinases reduces ischemia-reperfusion acute kidney injury
Peritubular capillary (PTC) injury and interstitial hemorrhage before ischemia (control) (a–d) and 6 h (e–l) after 60-min ischemia-reperfusionin MMP-2+/+ mice. MMP-2+Lectin (a, e: × 600; i: × 800): double immunofluorescence studies of MMP-2 (FITC, green) and Tomato Lectin (Texas-red, red) showed expression of MMP-2 on endothelial cells in PTCs (arrowhead in e, i) and cells present around PTCs (probably pericytes or fibrocytes) (arrow in e, i). In situ zymography (b, j: × 600; f: × 100) (*) in (f) indicates the outer stripe of outer medulla in kidney. Gelatinase activity was evident in interstitium around tubules including PTCs in the outer medulla at 6 h after ischemia-reperfusion. TUNEL (c, g: × 600; k: × 800): TUNEL stain identified apoptotic cells with positive nuclear stain in the interstitium around renal tubules at 6 h after ischemia-reperfusion, before the development of tubular epithelial cell death. High magnification (k) clearly showed the TUNEL+apoptotic endothelial cell in PTC. Periodic acid-methenamine stain (d, h: × 600; l: × 800): interstitial hemorrhage around renal tubules at 6 h after 60-min ischemia-reperfusion, before the development of tubular epithelial cell necrosis. Arrow in (l) indicates PTCs with large and round endothelial cell nuclei in interstitial hemorrhage.
