Figure 4
From: Complementary inhibition of cerebral aneurysm formation by eNOS and nNOS
Expression of MCP-1 and VCAM-1 mRNA in the circle of Willis and subsequent macrophage infiltration in CA walls of eNOS−/− mice. Representative image of RT-PCR analysis for MCP-1 and VCAM-1 (a) and densitometric analysis (b, c) from five independent experiments. β-Actin served as an internal control. Before (0M) and 5 months (5M) after CA induction; n.s., statistically not significant. EvG staining (d, g), immunostaining for MCP-1 (green signal: e, h) and VCAM-1 (green signal: f, i) in CA walls of eNOS+/+ (e, f) and eNOS−/− mice (h, i). Bar=20 μm. The numbers of infiltrated macrophages in CA walls are shown in (j).
