Figure 5
STAT5b and Gab2 are required to observe IFNγ-induced increases in T84 epithelial monolayer macromolecular permeability. The bar graphs in (a, b) show that IFNγ (10 ng/ml; 48 h)+scrambled siRNA (Cont) resulted in significant increases in HRP fluxes and E. coli (HB101) translocation across confluent T84 monolayers that was blocked by use of STAT5b and Gab2 targeting siRNA (mean±s.d.; n=3 monolayers/treatment/experiment, representative of four experiments; *P<0.05 compared with control (Unstim); starting TER in these experiments ranged 1200–1600 Ohms/cm2). (c) Reduced expression of STAT5b and Gab2 protein in whole cell extracts of T84 cells treated with specific targeting siRNAs that was not observed in cells treated with an irrelevant scrambled (Cont) siRNA. (d) siRNA targeting Fyn or STAT5b reduced IFNγ-stimulated (2 h) membrane-associated activated Fyn in T84 cells. (c, d) Images are representative of three experiments. NS, nonspecific bands.
