Figure 2 | Laboratory Investigation

Figure 2

From: Novel guggulsterone derivative GG-52 inhibits NF-κB signaling in intestinal epithelial cells and attenuates acute murine colitis

Figure 2

Effects of the guggulsterone derivatives on chemokine expression in COLO 205 cell stimulated with TNF-α. COLO 205 cells were pretreated with 50 μM of the guggulsterone derivatives GG-46, GG-50B, GG-52, and GG-53 for 24 h and then stimulated with TNF-α (10 ng/ml) for 4 h. mRNA expression of IL-8 (a) and MCP-1 (b) was measured by real-time RT-PCR. Levels are normalized to β-actin. Data are expressed as fold change in mRNA transcript levels relative to the unstimulated control (mean±s.e.m., n=3). (c) COLO 205 cells were pretreated with the guggulsterone derivatives (50 μM) for 24 h and then stimulated with TNF-α (10 ng/ml) for 24 h. Secretion of IL-8 was measured by ELISA (mean±s.e.m., n=5). *P<0.05 compared with TNF-α alone.

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