Figure 4 | Laboratory Investigation

Figure 4

From: Novel guggulsterone derivative GG-52 inhibits NF-κB signaling in intestinal epithelial cells and attenuates acute murine colitis

Figure 4

Guggulsterone derivatives GG-50B and GG5 inhibit NF-κB DNA-binding activity in COLO 205 cells stimulated with TNF-α. (a) COLO 205 cells were pretreated with the guggulsterone derivatives GG-50B and GG-52 (10 or 50 μM) for 24 h and then stimulated with TNF-α (10 ng/ml) for 1 h. NF-κB DNA-binding activity in the nuclear extracts was assessed by EMSA. The data are representative of more than five independent experiments. (b) COLO 205 cells were transfected with 2 × pNF-κB-luciferase transcriptional reporter. After 24 h, the transfected cells were pretreated with the indicated concentrations of GG-50B or GG-52 for 24 h and were then combined with TNF-α (10 ng/ml) for 1 h. Data are expressed as mean-fold induction ±s.e.m. of luciferase activity relative to unstimulated controls (n=5). The mean fold induction of the β-actin reporter gene activity relative to the unstimulated controls remained relatively constant throughout each experiment. *P<0.05 compared with TNF-α alone.

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