Figure 5
Visualization of lung edema and imaging was performed within 5 min after FITC-dextran intravenous injection. (a) Normal lung showing interstitial microcirculatory network and moving blood cells negatively visible. (b) A 3-day hyperoxia-exposed lung showing heterogeneous diffusion of dextran outside microvessel network leading to interstitial and alveolar space labeling. *Shows alveolointerstitial edema (intermediate stage) and **shows massive, but localized, alveolar filling with stain-free microvessels. (c) Ex vivo labeling with H&E of normal lung. (d) Ex vivo labeling with H&E of hyperoxic lung with interstitial and alveolar edema.
