Table 1 Fluorescent marker's specifications, routes and targets for Five-1 lung imaging
Markers | Source | Way of administration | Dosage | Imaging targets |
|---|---|---|---|---|
FITC-Ricinus communis agglutinin-1 (RCA-1) | Vector Laboratories | I/T | 0.15 mg/ml in 5 ml saline | Type I and II epithelial cells Alveolar macrophages |
FITC-Pisum sativum agglutinin (PSA) | Vector Laboratories | I/T | 0.15 mg/ml in 5 ml saline | Some type I epithelial cells Alveolar macrophages |
FITC-labeled dextran 77 kDa | Sigma-Aldrich | I/V | 100–500 μl of 10 mg/ml saline | Lung edema and microcirculation |
Alexa Fluor 488-anti-RTI40 | Dr Dobbs Laboratory | I/T | 200 μl of 100 μg/ml | Type I epithelial cells |
FITC isotypic IgG1 from murine myeloma | Sigma-Aldrich | I/T | 200 μl of 100 μg/ml | None (control Abs) |
YO-PRO-1 | Molecular Probes | I/V or I/T | 1 ml of 50 μ M (I/V) 5 ml of 10 μM (I/T) | Apoptotic endothelial or epithelial cells |
PKH2 Green Fluorescent Cell Linker Kit for Phagocytic Cell Labeling | Sigma-Aldrich | I/V | 50 × 106 cells in 1 ml | Neutrophils |
Acriflavine | Sigma-Aldrich | I/V or I/T | 500 μl of 0.05% (I/V) 5 ml of 0.0025–0.05% (I/T) | Nucleus of endothelial or epithelial cells |
