Figure 1

(a) RT-PCR analysis for LRP1 and LRP2 in osteoblast cell lines. GAPDH was used to control. (b) Immunolocalization of bLF in ST2 cells at 4 h after bLF treatment. Scale bar: 10 μm. (c) Expression of TNF-α, RANKL, and OPG mRNAs in ST2 cells. Cells were harvested at various times (0–24 h) after 100 ng/ml A.a.-LPS stimulation, with 2-h-pretreatment (10 μg/ml) or without pretreatment (vehicle) of bLF. GAPDH was used to control. (d) Quantitative real-time RT-PCR analysis for TNF-α in osteoblast cell lines. GAPDH was used to control. Results are expressed as mean±s.d. ^**P<0.01. (e) Effect of bLF on in vitro TRAP+ cells formation. Primary osteoblasts and BMCs were co-cultured for 5 days. Co-cultures were incubated in the presence of A.a.-LPS (1 μg/ml) for the final 3 days. Pretreatment of bLF (1 or 10 μg/ml) significantly decreased the number of TRAP+ preosteoclasts and osteoclasts. Data are present as the means±s.d. (n=6 for each group). ^*P<0.05.