Table 2 Comparison of used amplification protocols to detect exon 23 skipping in the mdx mouse model

	This paper	Wu, 2009	Yin, 2008	Ivanova, 2008	Heemskerk, 2009	Lu, 2005	Vitiello, 2008	Doran, 2009	Denti, 2008
No. of cycles	30	40	(25+25)	(25+25)	(19+32)^a	(30+25)	(30+30)^a	(35+30)	(40+30)^b
RNA template ng in RT reaction	400	100	200	800	400	200	500	100	200
RNA template ng in PCR	30	100	200	800	60	200	50	100	200
No. of transcripts	665	2215	4430	17721	1329	4430	1108	2215	4430
Compared with 30 cycles	1	1024	1.E+06	1.E+06	2.E+06	3.E+07	1.E+09	3.E+10	1.E+12

Nanograms of total RNA template used for RT reaction and primary PCR (when different) are shown. The number of transcripts used as templates for the primary PCR as template is also shown. The last row shows the additional amount of amplification compared with the 30 cycles protocol used in this report.
^aBefore nested amplification DNA template was diluted 10 times.
^b4 μl of DNA template were used in nested PCR.

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