Figure 1

Gstk1 gene knockout construct. Panel (a) shows the organization of the BALB/c mouse Gstk1 gene and the DNA construct made in pKO Scrambler to inactivate the gene in BALB/c ES cells. The relative positions of the genotyping primers are shown in panel (a) and their sequence is listed in panel (b). Panel (c) shows a typical PCR genotyping experiment.