Table 2 Changes in glomerular basement membrane ultrastructure, foot process effacement and immune deposits with age
Age | 3 mo | 5–6 mo | 15 mo | |
|---|---|---|---|---|
Males | ||||
Glomerular basement membrane: morphological changes (0–3) | ||||
Wild type | − | − | + | |
Gstk1 −/− | ++ | +++ | +++ | |
Glomerular basement membrane: thickness (nm) (mean (s.d.)) | ||||
Wild type | 110.0 (7.0) | 111.2 (7.1) | 115.0 (7.4) | |
Gstk1 −/− NO | 111.3 (7.2) | 112.3 (7.6) | 116.3 (7.5) | |
Gstk1 −/− AB | 223.5 (21.4) | 240.1 (17.1) | 239.2 (18.2) | |
Foot process effacement: % (mean (s.d.)) | ||||
Wild type | 18.1 (5.7) | 10 (3.2) | 23.4 (12.9) | |
Gstk1 −/− | 23.7 (6.2)* | 47.5 (5.8)** | 39.7 (4.3)** | |
n | Wild type | 5 | 7 | 2 |
n | Gstk1 −/− | 5 | 7 | 2 |
Females | ||||
Glomerular basement membrane: morphological changes (0–3) | ||||
Wild type | − | − | (+) | |
Gstk1 −/− | + | + | ++ | |
Glomerular basement membrane: thickness (nm) (mean (s.d.)) | ||||
Wild type | 105.4 (6.6) | 107.6 (7.5) | 111.0 (8.2) | |
Gstk1 −/− NO | 105.8 (8.7) | 107.6 (7.5) | 111.9 (8.2) | |
Gstk1 −/− AB | 217.4 (23.5) | 231.6 (20.9) | 239.6 (24.0) | |
Foot process effacement: % (mean (s.d.)) | ||||
Wild type | 17.8 (5.5) | 10.0 (4.1) | 37.8 (2.6) | |
Gstk1 −/− | 23.4 (6.0)* | 39.7 (4.3)** | 29.7 (4.3)** | |
n | Wild type | 5 | 5 | 2 |
n | Gstk1 −/− | 5 | 5 | 2 |
Immune deposits (C3, IgM)—males and females | ||||
Electron microscopy | ||||
Wild type | + | ++ | +++ | |
Gstk1 −/− | + | ++ | +++ | |
Immunofluorescence | ||||
Wild type | + | ND | +++ | |
Gstk1 −/− | + | ND | +++ | |
