Figure 5
Knockdown of galectin-3 by siRNA transfection and galectin-1-induced cell death. (a) Galectin-3 siRNA treatment for 24 h significantly inhibited galectin-3 mRNA expression, as revealed by real-time RT-PCR analysis. HPRT-normalized relative expression of galectin-3 mRNA is indicated. *P=0.00108, the P-value was calculated by Student's t-test. Data represent an average±s.d. from three independent experiments. (b) Western blot analysis of galectin-3 siRNA-mediated knockdown of galectin-3 expression at 48 h of transfection (Galectin-3 runs at about 30 kDa). β-Actin was used as endogenous control (β-actin runs at about 40 kDa). Depicted data are representative from two independent experiments. (c) Based on flow-cytometric analysis, CD30-mediated enhancement of galectin-1-induced cell death was not altered by galectin-3 expression. Knockdown of galectin-3 by siRNA could not alter the amount of galectin-1-induced cell death with or without CD30 stimulation of Karpas 299 cells siRNA (NS: not significant). The statistical significant was determined by Student's t-test. Data represent an average of two independent experiments and bars represent standard deviation.
