Figure 3 | Laboratory Investigation

Figure 3

From: Heparin-binding EGF-like growth factor protects intestinal stem cells from injury in a rat model of necrotizing enterocolitis

Figure 3

Ex vivo crypt-villous organoid cultures. (a) A representative mouse crypt cultured ex vivo in gelmatrix that grew into a crypt-villous organoid with budding crypts (black arrows) extending from the surface at days 1–11. (b) Organoid illustration showing lumen, villus domain, crypt domain and ISCs in green (from Sato et al28). (c) Exponential growth of crypt-villous organoids cultured with R-spondin 1, Noggin and HB-EGF (50 ng/ml).

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