Table 1 RT-PCR primers for analysis

From: Blockade of IL-6 signaling exacerbates liver injury and suppresses antiapoptotic gene expression in methionine choline-deficient diet-Fed db/db mice

Genes

Direction

Sequences

Gus

Forward

5′-GCAGTTGTGTGGGTGAATGG-3′

 

Reverse

5′-GGGTCAGTGTGTTGTTGATGG-3′

SOCS3

Forward

5′-GGGTGGCAAAGAAAAGGAG-3′

 

Reverse

5′-GTTGAGCGTCAAGACCCAGT-3′

IL-6 Rα

Forward

5′-CATCTGTGAGTGGCGTCCGA-3′

 

Reverse

5′-AACGCTTCGTTGTGGCTGGA-3′

GP130

Forward

5′-AGCATCTCCAGCAACGAGGAG-3′

 

Reverse

5′-GGACAGGCTTCAGGCTGACT-3′

TNF-α

Forward

5′-TCGTAGCAAACCACCAAGTG-3′

 

Reverse

5′-AGATAGCAAATCGGCTGACG-3′

TGFβ-1

Forward

5′-TTGCCCTCTACAACCAACACAA-3′

 

Reverse

5′-GGCTTGCGACCCACGTAGTA-3′

Pro Col1α1

Forward

5′-GACATCCCTGAAGTCAGCTGC-3′

 

Reverse

5′-TCCCTTGGGTCCCTCGAC-3′

α-SMA

Forward

5′-ACTGGGACGACATGGAAAAG-3′

 

Reverse

5′-AGAGGCATAGAGGGACAGCA-3′

TIMP-1

Forward

5′-CATGGAAAGCCTCTGTGGATATG-3′

 

Reverse

5′-GATTGTGCAAATTTCCGTTCCTT-3′

FATP1

Forward

5′-CGCTTTCTGCGTATCGTCTG-3′

 

Reverse

5′-GATGCACGGGATCGTGTCT-3′

FATP2

Forward

5′-GGTATGGGACAGGCCTTGCT-3′

 

Reverse

5′-GGGCATTGTGGTATAGATGACATC-3′

FATP5

Forward

5′-CTACGCTGGCTGCATATAGATG-3′

 

Reverse

5′-CCACAAAGGTCTCTGGAGGAT-3′

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