Figure 4 | Laboratory Investigation

Figure 4

From: Role of amino acid transporter LAT2 in the activation of mTORC1 pathway and the pathogenesis of crescentic glomerulonephritis

Figure 4

Effect of everolimus on activation of S6 ribosomal protein in crescentic glomerulonephritis (CGN) glomerulus. (a) Immunohistochemistry and periodic acid-Schiff (PAS) staining examined by using serial sections revealed apparent reduction of phospho-S6 ribosomal protein in the crescent lesions (arrow) of everolimus-treated CGN. (b) Isolated glomeruli by using magnetic beads without collagenase treatment. Arrow indicates Bowman's capsule and arrowhead indicates crescents, respectively. (c) Western blot analysis by using protein samples from isolated glomeruli. Phospho-S6 ribosomal protein and vascular endothelial growth factor (VEGF) of the glomeruli from CGN (day 7) are strongly increased compared with that of control, whereas everolimus clearly abrogated upregulation of both proteins of CGN glomeruli. β-Actin shows internal control of each sample.

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