Figure 7

(a and b) Expression of L-type neutral amino acid transporter (LAT)2 and 4F2hc in the glomeruli of control and anti-glomerular basement membrane (GBM) glomerulonephritis rats. (a) Immunohistochemistry of LAT2 and 4F2hc in the glomerulus of control, day 2 and day 7 after injection of anti-GBM antibody. Larger insets show a higher magnification of the smaller insets. Scale bar=10 μm. (b) RT-PCR of mRNAs encoding LAT2 and 4F2hc from isolated glomeruli and the rest of the kidney of control, day 2 and day 7. β-Actin was used as an internal control.