Figure 9 | Laboratory Investigation

Figure 9

From: Role of amino acid transporter LAT2 in the activation of mTORC1 pathway and the pathogenesis of crescentic glomerulonephritis

Figure 9

(a and b) Amino acid starvation assay shown by western blot analysis with the activation of mammalian target of rapamycin complex 1 (mTORC1) pathway, 70S6K and 4E-BP1, in the absence or presence of L-type neutral amino acid transporter (LAT)-specific inhibitor BCH. Histograms show the comparison for the level of activation of both molecules, expressed by the amount of phosphorylated form over total protein. (a) Activation of p70S6K occurred at 30 min after nutrition supply and was sustained for 120 min. BCH clearly abrogated p70S6K activation. (b) Activation of 4E-BP1 was determined as a similar pattern to p70S6K.

Back to article page