Figure 3 | Laboratory Investigation

Figure 3

From: Pitavastatin-induced angiogenesis and arteriogenesis is mediated by Notch1 in a murine hindlimb ischemia model without induction of VEGF

Figure 3

Pitavastatin induces Notch1 cleavage and Hes-1 expression through Akt. (a) Immunoblots showing the time course of pitavastatin (100 nmol/l)-induced Akt and Notch1 activation, VEGF, Notch1 ligand (Jagged-1) and Hes-1 expression in human umbilical vein endothelial cells (HUVECs). Activation of Akt was assessed by Ser473 phosphorylation of Akt (p-Akt) relative to the total Akt (t-Akt). Activation of Notch1 was determined by the amount of cleaved Notch1 (c-Notch1) relative to total Notch1 (t-Notch1). Representative blots of three independent experiments with similar results. (b) Quantitative data based on three independent experiments. *P<0.001 and #P<0.05, compared with the control (0 time point).

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