Figure 1

G-protein-coupled receptor 55 (GPR55) is expressed in cholangiocyte and cholangiocarcinoma cell lines. GPR55 levels were assessed in four cholangiocarcinoma cell lines as well as the non-malignant cholangiocyte cell lines H69 and HIBEC, by real-time polymerase chain reaction (PCR) (a) and immunoblotting (b). Data are expressed as average relative GPR55 mRNA expression (±s.e.m.) in each cell line compared with H69 cells after using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression as a loading control (n=3; a).