Figure 3

TNF-α and IL-1β decrease the levels of TNAP transcripts in ATDC5 chondrocytes. In ATDC5 cells, TNF-α at 1 ng/ml and/or IL-1β at 0.1 ng/ml decreased the levels of TNAP after 48 h of treatment, as measured by quantitative PCR. ^*P<0.05 as compared with untreated cells.